t7 maxi script kit (Thermo Fisher)
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t7 maxi script kit
T7 Maxi Script Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxi+script+t7+kit/pmc02758590-199-10-12
Average 86 stars, based on 1 article reviews
T7 Maxi Script Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxi+script+t7+kit/pmc02758590-199-10-12
Average 86 stars, based on 1 article reviews
t7 maxi script kit - by Bioz Stars,
2026-11
86/100 stars
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Synthesized:Article Title: Functional role of annexins in zebrafish caudal fin regeneration - A gene knockdown approach in regenerating tissue. Article Snippet: .. 2.2.sgRNA mRNA synthesis sgRNA mRNA specific to ANXA2a and ANXA2b targets were synthesized following DraI restriction digestion for eluting 282 bp fragment containing the T7 promoter and the sgRNA targets. sgRNA has been transcribed by in vitro transcription using the In Vitro:Article Title: Functional role of annexins in zebrafish caudal fin regeneration - A gene knockdown approach in regenerating tissue. Article Snippet: .. 2.2.sgRNA mRNA synthesis sgRNA mRNA specific to ANXA2a and ANXA2b targets were synthesized following DraI restriction digestion for eluting 282 bp fragment containing the T7 promoter and the sgRNA targets. sgRNA has been transcribed by in vitro transcription using the Article Title: Regulation of Apoptotic Endonucleases by EndoG Article Snippet: The nuclei were resuspended in 50 mM Tris-HCl, pH 7.9, 0.25 M sucrose, 1 mM CaCl 2 , and 5 mM 2-mercaptoethanol and exposed with varying concentrations of recEndoG or bleomycin (Sigma-Aldrich, St. Louis, MO) for 1 h. Total RNA was extracted and real-time RT-PCR was performed as described below. .. For in vitro transcription, recEndoG or bleomycin were exposed to mouse mature EndoG gene in pET29b vector (Novagen, Madison, WI) or linearized pTRI-β-actin-mouse plasmid (Ambion, Austin, TX), and Article Title: The Non-Coding RNA Ncr0700/PmgR1 is Required for Photomixotrophic Growth and the Regulation of Glycogen Accumulation in the Cyanobacterium Synechocystis sp. PCC 6803. Article Snippet: The separated RNA was transferred to a Biodyne 0.45 mm nylon membrane (PALL Life Sciences) by capillary blotting with 20 SSC (3 M NaCl, 0.3 M sodium citrate) overnight. .. After UV cross-linking, the membranes were hybridized with [a-32P]UTP-labeled, single-stranded RNA probes generated by in vitro transcription from PCR templates using the Recombinase Polymerase Amplification:Article Title: Modulation of Igf2 genomic imprinting in mice induced by 5-azacytidine, an inhibitor of DNA methylation. Article Snippet: As previously described (19), the RPA quantification of IGF-II mRNA was performed using RPA II kit (Ambion Inc., Austin, TX). .. The RPA probe for IGF-II was prepared by Plasmid Preparation:Article Title: Modulation of Igf2 genomic imprinting in mice induced by 5-azacytidine, an inhibitor of DNA methylation. Article Snippet: As previously described (19), the RPA quantification of IGF-II mRNA was performed using RPA II kit (Ambion Inc., Austin, TX). .. The RPA probe for IGF-II was prepared by Article Title: Regulation of Apoptotic Endonucleases by EndoG Article Snippet: The nuclei were resuspended in 50 mM Tris-HCl, pH 7.9, 0.25 M sucrose, 1 mM CaCl 2 , and 5 mM 2-mercaptoethanol and exposed with varying concentrations of recEndoG or bleomycin (Sigma-Aldrich, St. Louis, MO) for 1 h. Total RNA was extracted and real-time RT-PCR was performed as described below. .. For in vitro transcription, recEndoG or bleomycin were exposed to mouse mature EndoG gene in pET29b vector (Novagen, Madison, WI) or linearized pTRI-β-actin-mouse plasmid (Ambion, Austin, TX), and Clone Assay:Article Title: Modulation of Igf2 genomic imprinting in mice induced by 5-azacytidine, an inhibitor of DNA methylation. Article Snippet: As previously described (19), the RPA quantification of IGF-II mRNA was performed using RPA II kit (Ambion Inc., Austin, TX). .. The RPA probe for IGF-II was prepared by Polymerase Chain Reaction:Article Title: Genomic analysis of expressed sequence tags in American black bear Ursus americanus Article Snippet: .. RNA subtraction drivers were obtained from PCR product by using in vitro transcription with Article Title: The Non-Coding RNA Ncr0700/PmgR1 is Required for Photomixotrophic Growth and the Regulation of Glycogen Accumulation in the Cyanobacterium Synechocystis sp. PCC 6803. Article Snippet: The separated RNA was transferred to a Biodyne 0.45 mm nylon membrane (PALL Life Sciences) by capillary blotting with 20 SSC (3 M NaCl, 0.3 M sodium citrate) overnight. .. After UV cross-linking, the membranes were hybridized with [a-32P]UTP-labeled, single-stranded RNA probes generated by in vitro transcription from PCR templates using the In Vitro Transcription:Article Title: Genomic analysis of expressed sequence tags in American black bear Ursus americanus Article Snippet: .. RNA subtraction drivers were obtained from PCR product by using in vitro transcription with Generated:Article Title: The Non-Coding RNA Ncr0700/PmgR1 is Required for Photomixotrophic Growth and the Regulation of Glycogen Accumulation in the Cyanobacterium Synechocystis sp. PCC 6803. Article Snippet: The separated RNA was transferred to a Biodyne 0.45 mm nylon membrane (PALL Life Sciences) by capillary blotting with 20 SSC (3 M NaCl, 0.3 M sodium citrate) overnight. .. After UV cross-linking, the membranes were hybridized with [a-32P]UTP-labeled, single-stranded RNA probes generated by in vitro transcription from PCR templates using the |